The Use of Terminators and Anti - Terminators to Expand the Two - Spatula
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Search for additional replication terminators in the Bacillus subtilis 168 chromosome.
The Bacillus subtilis 168 chromosome is known to contain at least six DNA replication terminators in the terminus region of the chromosome. By using a degenerate DNA probe for the consensus terminator sequence and low-stringency hybridization conditions, several additional minor hybridizing bands were identified. DNA corresponding to the most intense of these bands was cloned and characterized....
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The nucleotide sequences of 30 factor-independent terminators of transcription with RNA polymerase from E. coli have been compiled and analyzed. The standard features - a stretch of thymine residues and a preceding dyad symmetry - are shared by most sequences, but there are striking exceptions which indicate that these features alone are not sufficient to describe these sites. In two thirds of ...
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The complex terminator region of the Escherichia coli rrnB gene was analyzed by subcloning the terminators T1 and T2 and the inverted repeats IR1 and IR2 individually, or in various combinations, in a normal or inverted orientation into a terminator probe vector. The in vivo terminating efficiency was assayed by measuring the galactokinase activity encoded by the downstream galK gene. Terminati...
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Recent work has shown that the ability of moving contour terminators to determine the perceived motion of untextured contours is strongly constrained by whether contour terminators are classified as intrinsic (belonging to a moving contour) or extrinsic (belonging to a surface occluding a moving contour). It has also been demonstrated that stereopsis can play a decisive role in this classificat...
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A new algorithm called RNAMotif containing RNA structure and sequence constraints and a thermodynamic scoring system was used to search for intrinsic rho-independent terminators in the Escherichia coli K-12 genome. We identified all 135 reported terminators and 940 putative terminator sequences beginning no more than 60 nt away from the 3'-end of the annotated transcription units (TU). Putative...
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